TABLE 2.
Subunit quantification in holo-Na+-NQR by Edman degradation
| Cycle | NqrA | NqrB | NqrC | NqrD | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aaa | pmolb | aaNqrA/aaNqrDc | aa | pmol | aaNqrB/aaNqrD | aa | pmol | aaNqrC/aaNqrD | aa | pmol | aaNqrD/aaNqrD | |
| 3 | S | 10.06 | 0.44 | K | 19.29 | 0.85 | N | 12.64 | 0.56 | A | 22.62 | 1.00 |
| 5 | H | 6.67 | 0.39 | F | 23.60 | 1.38 | D | 18.39 | 1.08 | E | 17.07 | 1.00 |
| 7 | H | 6.74 | 0.40 | E | 16.95 | 1.01 | I | 16.14 | 0.96 | K | 16.74 | 1.00 |
| 9 | H | 7.76 | 0.73 | I | 16.74 | 1.58 | K | 25.53 | 2.41 | S | 10.58 | 1.00 |
| 10 | S | 7.06 | 0.30 | E | 16.83 | 0.72 | T | 11.49 | 0.49 | V | 23.40 | 1.00 |
| 12 | G | 11.89 | 0.52 | H | 9.19 | 0.41 | F | 14.95 | 0.66 | A | 22.67 | 1.00 |
| Mean | 0.47 | 0.99 | 1.03 | 1.00 |
a aa, amino acid residue present at that position as derived from the corresponding nqr gene on plasmid pNQR1.
b Amount normalized against the standard PTH amino acid mixture, not corrected for the sequencing yield is shown.
c The ratio between the amount of amino acid at this position of the given subunit and the amount of the amino acid at the corresponding position in subunit NqrD is shown.