TABLE 1.
The 61 T-DNA insertion lines of PPR proteins investigated are listed in the SALK collection as being homozygous
Lines are ordered according to the genomic locus in the Arabidopsis genome. The subclass characterized by the C-terminal extension is given in the next column. 58 of the PPR proteins analysed contain an E-domain, 32 of these also a DYW extension. For controls, 3 PLS-PPR proteins were included. Predictions by the two target analysis programs TargetP and predotar are given in the following columns: C indicating a plastid (chloroplast) and M a mitochondrial prediction. Names of genes with known functions are given in bold for those newly found in this investigation, and their target sites are identified. Citations are given for those identified by others before or during the course of this work. The last two columns give the SALK numbers, and show the location of the T-DNA insert.
| Arabidopsis locus | Subclass | TargetP | Predotar | Gene name | Editing defect in mitochondria (position in CDS) | Editing defect in Chloroplast (genome position) | Refs. | T-DNA insertion lines | T-DNA location |
|---|---|---|---|---|---|---|---|---|---|
| At1g04840 | DYW | M | SALK_020491C | CDS | |||||
| At1g08070 | DYW | C | C | SALK_084804C | CDS | ||||
| At1g29710 | DYW | M | M | SALK_102463C | Promoter | ||||
| At1g47580 | E+ | M | M | SALK_038140C | Promoter | ||||
| At1g50270 | E+ | SALK_036097C | CDS | ||||||
| At1g59720 | DYW | C | CRR28 | ndhB (96698), ndhD (116290) | SALK_115133C | CDS | |||
| At1g64310 | E | M | C | SALK_069611C | CDS | ||||
| At1g68930 | DYW | M | SALK_085526C | Promoter | |||||
| At1g71420 | DYW | C | M | SALK_083202C | CDS | ||||
| At1g71490 | PLS | C | SALK_132831C | CDS | |||||
| At1g74600 | E | C | C | SALK_112173C | Promoter | ||||
| At2g20540 | E+ | MEF21 | cox3-257 | SALK_123212C | CDS | ||||
| At2g25580 | DYW | M | M | MEF8 | nad5-676 | SALK_106391C | CDS | ||
| At2g27610 | DYW | M | SALK_067071C | CDS | |||||
| At2g29760 | DYW | C | C | OTP81 | rps12 intron (69553) | Hammani et al. (42) | SALK_092402C | CDS | |
| At2g40720 | E+ | SALK_039787C | CDS | ||||||
| At3g02330 | E+ | M | C | SALK_097270C | CDS | ||||
| At3g03580 | DYW | C | C | SALK_025107C | Promoter | ||||
| At3g05240 | E | M | MEF19 | ccb206-566 | SALK_131274C | CDS | |||
| At3g11460 | DYW | M | M | SALK_061966C | Promoter | ||||
| At3g12770 | DYW | MEF22 | nad3-149 | SALK_107216C | CDS | ||||
| At3g14330 | DYW | M | SALK_077977C | CDS | |||||
| At3g14730 | E+ | M | C | SALK_093086C | Promoter | ||||
| At3g18970 | E | M | M | MEF20 | rps4-226 | SALK_051287C | CDS | ||
| At3g22690 | DYW | C | C | YS1 | rpoB (25992) | Zhou et al. (29) | SALK_123515C | CDS | |
| At3g24000 | DYW | M | M | SALK_055539C | 5′UTR | ||||
| At3g25970 | E+ | S | SALK_070881C | Promoter | |||||
| At3g26630 | PLS | C | C | SALK_124331C | 5′UTR | ||||
| At3g46790 | DYW | C | CRR2 | Hashimoto et al. (41) | SALK_030786C | CDS | |||
| At3g50420 | E+ | SALK_062063C | CDS | ||||||
| At3g51320 | E | M | M | SALK_022718C | 5′UTR | ||||
| At4g01990 | E | M | M | SALK_088721C | CDS | ||||
| At4g14050 | DYW | M | M | SALK_052078C | CDS | ||||
| At4g14820 | DYW | C | SALK_128858C | CDS | |||||
| At4g15720 | DYW | SALK_089468C | Promoter | ||||||
| At4g18520 | PLS | C | M | SALK_054374C | CDS | ||||
| At4g18750 | DYW | C | C | SALK_106218C | Promoter | ||||
| At4g19220 | E | M | M | SALK_032609C | Promoter | ||||
| At4g20770 | E | M | M | SALK_061964C | CDS | ||||
| At4g22760 | E | S | M | SALK_046917C | Promoter | ||||
| At4g30700 | DYW | M | SALK_059311C | CDS | |||||
| At4g32450 | DYW | M | M | SALK_047005C | CDS | ||||
| At4g33170 | DYW | C | SALK_043048C | CDS | |||||
| At4g35130 | DYW | C | C | SALK_0649 49C | CDS | ||||
| At4g37170 | DYW | M | SALK_021806C | Promoter | |||||
| At4g38010 | E | SALK_062576C | CDS | ||||||
| At4g39530 | E+ | M | M | SALK_130673C | 3′UTR | ||||
| At5g15340 | DYW | M | M | SALK_033610C | CDS | ||||
| At5g16860 | DYW | M | SALK_126201C | Promoter | |||||
| At5g19020 | E | M | M | MEF18 | nad4-1355 | SALK_126644C | CDS | ||
| At5g27110 | E+ | M | C | SALK_053627C | CDS | ||||
| At5g43790 | E | SALK_064501C | CDS | ||||||
| At5g46460 | DYW | M | M | SALK_033891C | 5′UTR | ||||
| At5g47460 | E | M | SALK_079594C | CDS | |||||
| At5g50390 | DYW | C | C | SALK_076606C | Promoter | ||||
| At5g52850 | DYW | C | C | SALK_085242C | CDS | ||||
| At5g59200 | E+ | C | C | OTP86 | rps14(37161) | Hammani et al., (42) | SALK_060533C | CDS | |
| At5g59600 | E | SALK_072473C | 5′UTR | ||||||
| At5g61800 | E | M | M | SALK_109464C | CDS | ||||
| At5g66500 | E | M | M | SALK_044034C | CDS | ||||
| At5g66520 | DYW | SALK_078415C | CDS |