FIGURE 1.

RT-PCR and Western blot detection of matriptase in glioma cell lines and fresh GBM tissues. A, agarose gels of RT-PCR products detect matriptase RNA in SKMG and U251MG cell lines, freshly resected GBM, and anaplastic astrocytoma (AA). A matriptase message is not detected in primary human astrocytes or fresh astrogliosis tissue. No RNA in the RT-PCR reaction is used as a negative control. B, Western blot of whole-cell lysates with a matriptase antibody shows that expression of matriptase in glioma cell lines (D54MG, U251MG, and U87MG) and two different human primary GBM is higher than its expression in two different primary human astrocytes. The blot was probed for actin as a loading control.