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. 2010 Jul 2;285(35):27130–27143. doi: 10.1074/jbc.M110.153213

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Detection of cleavage fragments of HA-tagged ASIC1 but not HA-tagged ASIC2 in Xenopus oocytes. A and C, normalized I_pH4.0 of oocytes injected either with ASIC1-HA (A) or ASIC2-HA (C) with or without 8 or 12 ng of matriptase cRNA. Matriptase decreases the peak I_pH4.0 of ASIC1-HA but not that of ASIC2-HA. Data are means ± S.D. The numbers of oocytes measured are shown in parentheses. The p values were determined with unpaired two-tailed t tests. B and D, consistent with the effect of matriptase on the function of the channels, Western blotting of whole oocyte lysates with an HA antibody detects fragments of ASIC1-HA (B) but not ASIC2-HA (D), suggesting that matriptase does not cleave ASIC2. The schematic of ASIC1-HA (below A and B) shows the locations of possible cleavage sites in the construct and the predicted fragment sizes. The black boxes represent the two transmembrane domains.