FIGURE 5.
Enzyme inhibitors do not alter TRPV4 mechanosensitivity in excised patches. Five patches were excised from each of two R616Q-expressing oocytes held at +50 mV before and another 5 × 2 patches excised after 30 min of incubation in the presence of 200 μm BPB (upper panels). a and b show sample traces of the response to suctions. c shows nPo increases by 60-mm Hg suctions in five different patches from one oocyte (black lines) and five from a second oocyte (gray lines) before the BPB treatment. d shows nPo increases by the same suctions of five new patches from the first oocyte (black lines) and five others from the second oocyte (gray lines) sampled after the BPB treatment. e summarizes the -fold increase in nPo from 0- to 60-mm Hg suction. (Those with nPo ∼ 0 without suction are excluded in the calculation.) f–j show parallel experimental results with 100 μm 17-octadecynoic acid (17-ODYA).