FIGURE 7.
Detection of nucleolin and AUF1 in MV-4-11 extracts and in UV-cross-linked immunoprecipitants. A, left panel, cytoplasmic extracts were separated by SDS-PAGE followed by Western blotting. Proteins were detected with anti-nucleolin antibody. A, right panel, cytoplasmic extracts were incubated with [32P]AREbcl-2 transcripts, exposed to UV light, and then digested with RNase. Immunoprecipitation was performed with anti-nucleolin antibody. Proteins were separated by SDS-PAGE, and the gel was dried and analyzed by phosphorimaging. B, left panel, same as described for A, except that proteins were detected with anti-AUF1 antibody. B, right panel, Same as described for A, except that immunoprecipitation was performed with anti-AUF1 antibody.