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. 2010 Jun 24;285(35):27192–27200. doi: 10.1074/jbc.M110.127118

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FIGURE 2. — Analysis of natural Amb a 4. A, MALDI-TOF MS analysis of natural ragweed Amb a 4 in comparison with mugwort Art v 1. The arrow indicates the calculated mass of the (large) isoform 1. The mass difference resulting from glycosylation of Amb a 4 is indicated. Depending on the prevailing isoforms this difference may even be larger. B, reducing SDS-PAGE of natural (n) and recombinant (r) Amb a 4. The arrow points at the polypeptide representing the complete amino acid sequence of Amb a 4 (isoform 1 with N-terminal His-tag), whereas the bands below are C-terminally truncated versions.