FIGURE 4.

17β-Estradiol stimulates PMCA-mediated Ca²⁺ efflux from hepatocytes. Ca²⁺ efflux from populations of intact rat hepatocytes was measured at 37 °C using fura-2 pentapotassium salt in the extracellular medium. The traces shown (A–F) are representative of several experiments. 300 μm caloxin, 10 nm water-soluble estradiol, 10 nm 17β-estradiol-BSA, or 10 nm 17α-estradiol were added where indicated by the arrows. Calculated mean rates of Ca²⁺ efflux of at least six experiments from at least three independent hepatocyte preparations are shown in G. H shows dependence of Ca²⁺ efflux stimulation on concentration of 17β-estradiol. *, significantly differently from control.