FIGURE 2.
Dynamic monitoring of cell adhesion to LN, CL type I, CL type IV, FN, or PLL-coated surfaces. A, GD3+ cells (G5 and G11) and control cells (V4 and V9) were seeded in the wells of 96-well e-plates at 104 cells/well with FCS, and cell attachment and spreading were monitored by the RT-CES system. The e-plates were pre-coated with LN (a), CL type I (b), CL type IV (c), FN (d), or PLL (e) as described under “Experimental Procedures.” B, similar experiments were performed without FCS in wells.