FIGURE 3.

CD spectra of 50 μm PFL activated by 0, 5, 5, 10, 20, 30, 40, 50, and 100 μm PFL-AE in the presence of 500 μm AdoMet, 100 μm 5-deazariboflavin, 100 mm Tris-HCl, pH 7.6, 100 mm KCl, 10 mm oxamate, and 10 mm DTT for 2 h. Each is a difference spectrum of PFL in activation mix minus the same activation mix lacking PFL. The numbers shown for each difference spectrum correspond to the PFL:PFL-AE ratios. The inset shows the contribution of PFL-AE to the CD spectra on the same scale as the main figure. Solid line is PFL-AE at 50 μm, and dashed line is PFL-AE at 1 μm. CD parameters: wavelength range, 170–300 nm; scan speed, 50 nm/min; data pitch, 0.1 nm; bandwidth, 0.5 nm; four scans accumulated.