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PFL activity is affected by the presence of PFL-AE
| PFL-AE:PFL | Ill. Timea | [Gly•]b | Activityc |
|---|---|---|---|
| min | μm | units/nmol | |
| 1:1 | 30 | 98 (±10) | 6.6 (±1) |
| 1:1 | 120 | 23 (±10) | NDd |
| 1:10 | 120 | 105 (±10) | 22 (±2) |
| 1:100 | 120 | 102 (±10) | 23 (±2) |
a Ill. Time is the time allowed for activation of PFL under photoreduction conditions.
b Glycyl radical was determined by EPR spin quantification.
c PFL activity is defined as 1 unit = 1 μmol of pyruvate turnover/min.
d Not determined; activity was below our limits of detection.
