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NMR titration analysis reveals that SCR1 and SCR2 of CR2 are both involved in ligating IFNα. Five superimposed 1H-15N TROSY-HSQC spectra of 15N-labeled CR2 SCR1–2 (0.6 mm in ⅓× PBS) were collected during titration with increasing amounts of IFNα. Black, no IFNα; blue, with 100 μm IFNα; purple, with 200 μm IFNα; green, with 400 μm IFNα; red, with 800 μm IFNα.
