FIGURE 8.

Knockdown of Dyn2 impedes degradation of the mature hERG channels in 0 mm K⁺ MEM. A, expression levels of Dyn1 or Dyn2 in hERG-HEK cells. The membrane was first probed with an anti-Dyn1 antibody. The same membrane was stripped and reprobed with an anti-Dyn2 antibody. IB, immunoblot. B, hERG expression levels in hERG-HEK cells transfected with scrambled (control (Ctl)) or Dyn2 siRNA after 6 h of culture in 5 or 0 mm K⁺ MEM. C, I_hERG in hERG-HEK cells transfected with scrambled or Dyn2 siRNA after 6 h of culture in 5 or 0 mm K⁺ MEM (upper panel). The averaged tail current amplitudes are summarized in the lower panel. The numbers in parentheses indicate the number of cells tested. **, p < 0.01.