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. 2010 Jul 6;285(35):27259–27264. doi: 10.1074/jbc.M110.124909

FIGURE 9.

FIGURE 9.

Knockdown of Cav3 slows the reduction of IKr in neonatal ventricular myocytes in low K+ conditions. A, co-immunoprecipitation of IKr and Cav3. Neonatal rat ventricular myocytes were lysed, and samples of 0.5 mg of protein were used for immunoprecipitation (IP) using an anti-Cav3 antibody. The immunoprecipitated protein was then immunoblotted (IB) using an anti-hERG antibody. B, knockdown of Cav3 using Cav3 siRNA in neonatal rat ventricular myocytes. C, IKr in neonatal rat ventricular myocytes transfected with scrambled (control (Ctl)) or Cav3 siRNA after 6 h of culture in 5 or 0.2 mm K+ MEM (upper panel). The averaged tail current amplitudes are summarized in the lower panel. The numbers in parentheses indicate the number of cells tested. **, p < 0.01.