TABLE 1.
Expression of MCT8 mRNA in various cancer cell lines
Cells were treated with or without tRA (1 μm) for 48 h, and quantitative RT-PCR of Mct8 and Gapdh was performed. For comparison of relative Mct8 expression among the cell lines, Mct8 expression was quantified with standard curves of Gapdh in each cell line and condition, and the average of F9 cells treated without tRA was set as 1. Values are means ± S.D. (n = 3).
| −tRA | +tRA | +tRA/−tRA | |
|---|---|---|---|
| F9 | 1.00 ± 0.08 | 77.4 ± 4.46a | 76.9 ± 8.32 |
| JEG3 | 2.03 × 10−4 ± 3.27 × 10−5 | 1.41 × 10−4 ± 8.50 × 10−5 | 0.74 ± 0.05 |
| MCF7 | 0.30 ± 0.01 | 0.51 ± 0.04 | 1.71 ± 0.19 |
| SH-SY5Y | 27.8 ± 3.34 | 20.5 ± 3.54 | 0.73 ± 0.04 |
a p < 0.01, when compared with untreated cells.