TABLE 2.
Expression of thyroid hormone transporter genes in F9 cells
Cells were treated with or without tRA (1 μm) for 96 h, and quantitative RT-PCR of the indicated genes was performed. For comparison among the genes tested, expression of each gene was quantified with standard curves of Gapdh from cDNA mixture of +tRA and −tRA. Values are means ± S.D. (n = 3). NA, not applicable.
| −tRA | +tRA | +tRA/−tRA | |
|---|---|---|---|
| Mct8 (Slc16a2) | 1.92 × 10−5 ± 1.29 × 10−6 | 1.29 × 10−2 ± 1.25 × 10−3a | 678.1 ± 78.6 |
| Mct10 (Slc16a10) | 1.51 × 10−4 ± 3.73 × 10−5 | 3.11 × 10−4 ± 2.78 × 10−5a | 2.05 ± 0.53 |
| Ntcp1 (Slc10a1) | <10−6 | <10−6 | NA |
| Lat1 (Slc7a5) | 7.26 × 10−2 ± 2.11 × 10−3 | 6.68 × 10−2 ± 2.08 × 10−3 | 0.96 ± 0.18 |
| Lat2 (Slc7a8) | 8.83 × 10−5 ± 5.51 × 10−6 | 4.08 × 10−4 ± 5.49 × 10-6a | 4.63 ± 0.21 |
| Oatp (Slco) 1a1 | 4.66 × 10−5 ± 5.23 × 10−7 | 3.37 × 10−5 ± 3.21 × 10−6 | 0.72 ± 0.05 |
| Oatp (Slco) 1a4 | <10−6 | <10−6 | NA |
| Oatp (Slco) 1a5 | <10−6 | <10−6 | NA |
| Oatp (Slco) 1b2 | 1.10 × 10−5 ± 6.45 × 10−7 | 5.20 × 10−6 ± 4.26 × 10−7 | 0.47 ± 0.05 |
| Oatp (Slco) 1c1 | <10−6 | <10−6 | NA |
| Oatp (Slco) 4a1 | 1.45 × 10−3 ± 9.48 × 10−5 | 2.35 × 10−4 ± 2.15 × 10−5 | 0.16 ± 0.002 |
| Oatp (Slco) 6b1 | <10−6 | <10−6 | NA |
| Oatp (Slco) 6c1 | <10−6 | <10−6 | NA |
| Gapdh | 1.29 ± 0.02 | 0.93 ± 0.14 | 0.72 ± 0.07 |
a p < 0.01, when compared with −tRA.