FIGURE 7.

Polysialylation of fully glycosylated NCAM is unaffected by the insertion of engineered glycans. Top panel, wild-type NCAM or NCAM with either the 443 or 465 glycosylation site inserted was immunoprecipitated from COS-1 cell lysates and incubated at 37 °C overnight either with (+) or without (−) PNGaseF enzyme. Proteins were resolved by SDS-PAGE, and polysialylation was measured using the anti-polysialic acid antibody, OL.28. Bottom panel, immunoblot analysis of relative NCAM protein expression levels.