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Effects of antibiotics on the binding activity of JadR2. Each lane contains 10 ng of labeled PjadR1. Lanes 2–12 contain 15 nm JadR2. Lanes 3 and 4 contain Me2SO and methanol as solvent controls, respectively. Lanes 5 and 6 contain 50 and 500 μm JdB, respectively. Lanes 7 and 8 contain 50 and 500 μm JdA, respectively. Lanes 9–12 contain Cm 5, 50, 500, and 750 μm, respectively.
