TABLE 1.

SIN3 isoforms vary in their ability to rescue lethality of genetic Sin3A loss of function alleles

Flies carrying one of two Sin3A alleles, either the UAS-187HA or UAS-220HA, or both transgenes and the tub-GAL4 transgene were generated and self-crossed. See “Experimental Procedures” for details. The number of progeny that were genetic null for Sin3A and that expressed neither, one, or both of the SIN3-tagged isoforms was determined. The percentage survival was calculated by setting the value for the number of Sin3A heterozygous flies expressing the indicated isoform(s) to 100% and comparing the number with that of the rescued Sin3A homozygous mutant flies expressing the indicated isoform(s). The results are the average of three independent experiments. S.E. is indicated.

Sin3A allele	Percentage survival
	Sin3A−/−	Sin3A+/−, 187HA+	Sin3A−/−, 187HA+
	%	%	%
P-element	0	100	6 ± 0.5
EMS	0	100	6 ± 1.3

	Sin3A−/−	Sin3A+/−, 220HA+	Sin3A−/−, 220HA+
P-element	0	100	74 ± 1.5
EMS	0	100	66 ± 6.3

	Sin3A−/−	Sin3A+/−, 187HA+, 220HA+	Sin3A−/−, 187HA+, 220HA+
P-element	0	100	88 ± 4.7
EMS	0	100	81 ± 1.9