FIGURE 8.

The expression of pro- or anti-apoptotic proteins (i.e. BAX, Bcl-2, and Bcl-X_L) or the mitochondrial membrane potential were not affected by transfections with siRNA^S100B or siRNA^scrambled (±UV) in either C8146A or SK-MEL-28 cells. A, Western blots of C8146A (wild-type p53, lanes 1–4) and SK-MEL-28 (^L145Rp53, lanes 5–8) melanoma cells transfected with either siRNA^S100B or siRNA^scrambled (±UV) using antibodies specific for BAX, Bcl-2, and Bcl-X_L proteins. To calculate the relative protein levels (i.e. -fold changes), blots were analyzed using densitometry and normalized to cells transfected with scrambled siRNA and not UV treated (i.e. -fold = 1.0), with actin blots on the same blot used as loading controls. B, line graphs illustrating no change in the ratio of BAX: Bcl-2 proteins (◇, open diamonds) or the ratio of BAX:Bcl-X_L proteins (▵, open triangles) as analyzed from Western blots of C81461A cells and SK-MEL-28 cells transfected with siRNA^scrambled or siRNA^S100B (±UV; 14 J/m²). C, no changes in the mitochondrial membrane potential (i.e. little if any monomeric JC-1) were observed in C81461A or SK-MEL-28 cells transfected with siRNA^scrambled or siRNA^S100B (±UV). Cells that were not transfected with siRNA (±UV) were included as additional negative controls and C8146A and SK-MEL-28 cells treated with valinomycin alone were included as positive controls.