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. Author manuscript; available in PMC: 2010 Aug 31.
Published in final edited form as: Trends Neurosci. 2002 Oct;25(10):518–524. doi: 10.1016/s0166-2236(02)02241-5

Fig. 2.

Fig. 2

Reproducing the effects of stress, administration of corticotropin-releasing hormone (CRH) to immature (10-day-old) rat induces production of Fos protein (black reaction product; arrows) in select populations of neurons in the amygdala (a,b) and hippocampus (c) that bear the CRF1 subtype of CRH receptor (brown immunoreactivity). Coronal sections from animals perfused 2 h after CRH treatment were double-labeled for Fos and CRF1 using standard methods [37,40,61]. CRH induced Fos production (arrows) preferentially in the central nucleus of the amygdala (ACe) (a, b) and in CA3a hippocampal pyramidal cell layer (c). Higher magnification (b) demonstrates cells of the amygdala double-labeled for the nuclear protein Fos and the membranous CRF1. Scale bar, 100 μm for (a,c), 20 μm for (b). Abbreviation: BL, Basolateral nucleus of the amygdala.