Fig. 5.
Adenosine triphosphate–sensitive K+ (KATP) currents in whole cell recordings are not affected by specific blockers of mitochondrial KATP (mito K-ATP) channels. (A) Whole cell recording demonstrating the lack of effect of the mito K-ATP channel blocker 5-hydroxy-decanoic acid (5-HD, 0.5 mm) on KATP channels made up of Kir6.2 and SUR1. In contrast, tolbutamide (Tb) strongly and reversibly inhibited the whole cell current. The drugs were applied as indicated by the bars above the recording. (B) Current–voltage relations in the absence (control) and presence of 0.5 mm 5-HD or 0.1 mm tolbutamide. (C) Mean data from experiments as that shown in A. *P < 0.05.