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. 2010 Aug 31;5(8):e12488. doi: 10.1371/journal.pone.0012488

Figure 1. Generation of transgenic mice and primary mouse embryonic fibroblasts (pMEFs).

Figure 1

(A) Top panel: scheme of the plasmid constructs used for the generation of the L3 and L3R transgenic mouse lines. Lower panel: scheme of the cross between the transgenic mouse lines and isolation of pMEFs from E13.5 mouse embryos. (B) PCR and RT-PCR analysis of mouse embryos showing Leu3 expression specifically in embryos expressing L3 transgene. (C) RT-PCR analysis of pMEFs showing Leu3 expression in the cell lines isolated from L3 transgenic embryos and lack of GFP expression in L3R pMEFs when α-ΙΡΜ is not added in the culture. RT-PCR for GAPDH was used as a control. L3: Leu3 transgene/L3R: Leu3 reporter transgene/E1-5: embryo 1/M1-5: pMEF cell line 1 (scale bar 200μm).