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. 2010 Aug 31;4(8):e809. doi: 10.1371/journal.pntd.0000809

Figure 1. Actin is required for DV2 entry.

Figure 1

A. DV2 infection induces rearrangements of the actin filaments in ECV304 cells. Serum-starved ECV304 cells were incubated with DV2 at 37°C. Then cells were fixed at the indicated time points, and stained for F-actin with phalloidin-TRITC. (×1600). B. Dose and time-dependent inhibition of DV2 entry by actin inhibitors. ECV304 cells were incubated with DMEM containing different concentrations of Jas (100, 50, or 10 nM) or Cyt D (4, 2, or 0.2 µM) for 1 h, 3 h, or 5 h. As a control, cells were pretreated with 0.1% DMSO, a solvent for these two inhibitors. The cells were infected with DV2 in the presence or absence of inhibitors at 37°C for 1 hour. Then the cells were treated with acid glycine solution (pH 3.0) for 2 min at room temperature to inactivate extracellular viruses, washed twice with PBS, and collected. The titers of cell samples were measured by plaque assay with Vero cells. The titer of mock treatment (DMSO control) was considered as 100%. Experiments were performed in duplicate for at least three independent experiments. *P<0.05 vs. DMSO control.