FIG. 6.

Lipolysis in abdominal adipocytes isolated from treated and untreated SHROB rats in response to isoproterenol stimulation. The baseline period is indicated by shaded area, and downward arrow indicates onset of isoproterenol stimulation (10 μM for 4 min). Abdominal adipocytes were isolated by collagenase digestion and filtration. Identical 200-μL aliquots of adipocytes were perifused with oxygenated Krebs3 buffer containing 0.1% bovine serum albumin and 0.025% adenosine deaminase at a rate of 0.25 mL/min, and 4-min fractions were collected and analyzed for glycerol.