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Flow cytometric enumeration of frequency of CTAg responding CD8+ cells. Following the IFN-γ cytokine secretion assay and enrichment, perioheral blood mononuclear cells incubated with dimethylsulfoxide only during the stimulation period are shown, prior to (A) and following (C) magnetic selection, along with IFN-γ responses to MAGE-A2-212–220 prior to (B) and following (D) magnetic selection.
