Table I.
Biophysical properties of heteromeric Kv1 channels
| Parameters | Adjacent Kv1.1-1.1-1.2-1.2 | Reverse adjacent Kv1.2-1.2-1.1-1.1 | Diagonal Kv1.1-1.2-1.1-1.2 | Reverse diagonal Kv1.2-1.1-1.2-1.1 |
| τact at 0 mV (ms) | 1.9 ± 0.1 (3) | 2.3 ± 0.6 (3) | 1.4 ± 0.1 (3) | 1.3 ± 0.1 (4) |
| τinact1 at 0 mV (ms) | 540 ± 2 (5) | 529 ± 50 (4) | 306 ± 34 (3)a | 341 ± 17 (4)a |
| τinact2 at 0 mV | 6,200 ± 500 (5) | 5,053 ± 596 (4) | 3,586 ± 485 (3) | 4,372 ± 404 (4) |
| V 1/2 (mV) | −17 ± 1 (9) | −18 ± 1 (14) | −22 ± 1 (7)a | −21 ± 1 (10)a |
| Slope (k) | 11 ± 1 (9) | 12 ± 1 (14) | 11 ± 1 (7) | 10 ± 1 (10) |
Values (mean ± SEM) for activation and inactivation were derived by fitting to single- and double-exponential functions, respectively. V1/2 and slope k were calculated from Boltzmann equation fitting of the gk-V plots.
a
Significant difference from adjacent constructs (P < 0.05; Mann-Whitney U test).