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. 2010 Sep;136(3):325–338. doi: 10.1085/jgp.201010454

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© 2010 Royer et al.

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Figure 1. — Cytosolic [Ca²⁺] in different external solutions. Area-averaged ratio of fluorescence of indo-1 in patched cells immersed in reference external solution (red) or in 0 Ca (black) versus time after breaking into the cell. The dashed curve in black is an exponential fit. The continuous black line marks the average for cells in 0 Ca and in cyan is R_min, measured in situ, in cells exposed to a Ca²⁺-free solution, with membrane permeabilized by saponin. Individual cells are identified by different symbols. ID: red, 032910a, b, d; black, 0325a, c, d; black, 0330a, b, c.