Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 30;207(9):1825–1833. doi: 10.1084/jem.20100500

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Campbell et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — ERs differentially modulate fibroblast migration in vitro. (A) Fibroblast-mediated contraction of collagenous discs was inhibited by 17β-estradiol (E), ERβ agonist (DPN; 10-6M), or ERα agonist (PPT; 10-6M). (B) Immunohistochemical quantification of wound α-SMA. (C and D) E and DPN promoted fibroblast migration compared with untreated cells (−). Co-treatment with E and the ER antagonist (ICI; 10-7 M) blocked migration, whereas cotreatment with ERα antagonist (MMP; 10-5M) increased migration. PHTPP, ERβ antagonist (10-5M). (E) Fibroblast proliferation assay. Data shown as mean ± SEM using triplicate wells (A, C–E) or 5–7 animals per group (B) are representative of two independent experiments. Bars: (A) 400 µm; (D) 600 mm. *, P < 0.05; **, P < 0.01.