Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 30;207(9):1863–1870. doi: 10.1084/jem.20092514

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Kim et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Antibodies raised by the nontoxigenic SpA vaccine block the association of immunoglobulin and vWF with SpA. (A) Rabbit antibodies raised against SpA-D_KKAA were affinity purified on a matrix with immobilized antigen and analyzed by Coomassie blue–stained SDS-PAGE under reducing conditions (lane 1, α-SpA-D_KKAA). SpA-D_KKAA antibodies were treated with pepsin to separate Fc and F(ab)₂ (lane 2). The resulting F(ab)₂ fragments were repurified by affinity chromatography on SpA-D_KKAA (lane 3). (B and C) SpA-D_KKAA specific F(ab)₂ was added to wild-type SpA, SpA-D, or SpA-D_KKAA, and the association with human IgG (B; n = 3) or vWF (C; n = 3) was measured. Data are the means and error bars represent ±SEM. Results in A–C are representative of three independent analyses. *, P < 0.01.