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. 2010 Aug 30;207(9):1863–1870. doi: 10.1084/jem.20092514

Table I.

Active immunization of mice with SpA vaccines

Antigen Staphylococcal load and abscess formation in renal tissue
Log10 CFU g−1a P valueb Reduction (log10 CFU g−1)c IgG titerd Number of abscessese P-valueb
S. aureus Newman challenge
Mock 6.46 ± 0.25 N/A N/A <100 3.7 ± 1.2 N/A
SpA 3.95 ± 0.56 0.0003 2.51 1,706 ± 370 2.1 ± 1.2 0.3581
SpA-D 4.43 ± 0.41 0.0001 2.03 381 ± 27 1.5 ± 0.8 0.1480
SpA DKKAA 3.39 ± 0.50 <0.0001 3.07 5,600 ± 801 0.5 ± 0.4 0.0204
S. aureus USA300 (LAC) challenge
Mock 7.20 ± 0.24 N/A N/A <100 4.0 ± 0.8 N/A
SpA 6.81 ± 0.26 0.2819 0.39 476 ± 60 3.3 ± 1.0 0.5969
SpA-D 6.34 ± 0.52 0.1249 0.86 358 ± 19 2.2 ± 0.6 0.0912
SpA-DKKAA 6.00 ± 0.42 0.0189 1.20 3,710 ± 1147 1.6 ± 0.6 0.0277
SpAKKAA 3.66 ± 0.76 0.0001 3.54 10,200 ± 2476 1.2 ± 0.5 0.0109
a

Means ± SEM of staphylococcal load calculated as log10 CFU g−1 in homogenized renal tissues 4 d after infection in cohorts of 15–20 BALB/c mice per immunization. A representative of three independent and reproducible animal experiments is shown.

b

Statistical significance was calculated with the unpaired two-tailed Students t test and p-values were recorded.

c

Reduction in bacterial load calculated as log10 CFU g−1.

d

Means ± SEM of five randomly chosen serum IgG titers were measured before staphylococcal infection by ELISA using SpA-DKKAA or SpA-DGGSS as antigens.

e

Histopathology of hematoxylin-eosin–stained thin-sectioned kidneys from 10 animals. The mean number of abscesses per kidney was recorded and averaged again for the final mean ± SEM.