Table I.
Antigen | Staphylococcal load and abscess formation in renal tissue | |||||
Log10 CFU g−1a | P valueb | Reduction (log10 CFU g−1)c | IgG titerd | Number of abscessese | P-valueb | |
S. aureus Newman challenge | ||||||
Mock | 6.46 ± 0.25 | N/A | N/A | <100 | 3.7 ± 1.2 | N/A |
SpA | 3.95 ± 0.56 | 0.0003 | 2.51 | 1,706 ± 370 | 2.1 ± 1.2 | 0.3581 |
SpA-D | 4.43 ± 0.41 | 0.0001 | 2.03 | 381 ± 27 | 1.5 ± 0.8 | 0.1480 |
SpA DKKAA | 3.39 ± 0.50 | <0.0001 | 3.07 | 5,600 ± 801 | 0.5 ± 0.4 | 0.0204 |
S. aureus USA300 (LAC) challenge | ||||||
Mock | 7.20 ± 0.24 | N/A | N/A | <100 | 4.0 ± 0.8 | N/A |
SpA | 6.81 ± 0.26 | 0.2819 | 0.39 | 476 ± 60 | 3.3 ± 1.0 | 0.5969 |
SpA-D | 6.34 ± 0.52 | 0.1249 | 0.86 | 358 ± 19 | 2.2 ± 0.6 | 0.0912 |
SpA-DKKAA | 6.00 ± 0.42 | 0.0189 | 1.20 | 3,710 ± 1147 | 1.6 ± 0.6 | 0.0277 |
SpAKKAA | 3.66 ± 0.76 | 0.0001 | 3.54 | 10,200 ± 2476 | 1.2 ± 0.5 | 0.0109 |
Means ± SEM of staphylococcal load calculated as log10 CFU g−1 in homogenized renal tissues 4 d after infection in cohorts of 15–20 BALB/c mice per immunization. A representative of three independent and reproducible animal experiments is shown.
Statistical significance was calculated with the unpaired two-tailed Students t test and p-values were recorded.
Reduction in bacterial load calculated as log10 CFU g−1.
Means ± SEM of five randomly chosen serum IgG titers were measured before staphylococcal infection by ELISA using SpA-DKKAA or SpA-DGGSS as antigens.
Histopathology of hematoxylin-eosin–stained thin-sectioned kidneys from 10 animals. The mean number of abscesses per kidney was recorded and averaged again for the final mean ± SEM.