Table II.

Passive immunization of mice with antibodies against SpA

Antibodya	Staphylococcal load and abscess formation in renal tissue
	Log10 CFU g−1b	P valuec	Reduction (log10 CFU g−1)d	IgG titere	Number of abscessesf	P-valuec
Mock (α-V10)	7.10 ± 0.14	N/A	N/A	<100	4.5 ± 0.8	N/A
α-SpA-DKKAA	5.53 ± 0.43	0.0016	1.57	466 ± 114	1.9 ± 0.7	0.0235
α-SpAKKAA	5.69 ± 0.34	0.0005	1.41	1,575 ± 152	1.6 ± 0.5	0.0062

^aAffinity-purified antibodies were injected into the peritoneal cavity of BALB/c mice at a concentration of 5 mg × kg⁻¹ 24 h before intravenous challenge with 1 × 10⁷ CFU S. aureus Newman.

^bMeans ± SEM of staphylococcal load calculated as log₁₀ CFU g⁻¹ in homogenized renal tissues 4 d after infection in cohorts of 15 BALB/c mice per immunization. A representative of three independent and reproducible animal experiments is shown.

^cStatistical significance was calculated with the unpaired two-tailed Students t test and p-values were recorded.

^dReduction in bacterial load calculated as log₁₀ CFU g⁻¹.

^eMeans ± SEM of five randomly chosen serum IgG titers were measured before staphylococcal infection by ELISA.

^fHistopathology of hematoxylin-eosin–stained thin-sectioned kidneys from 10 animals. The mean number of abscesses per kidney was recorded and averaged again for the final mean ± SEM.