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. 2010 Aug 30;207(9):1907–1921. doi: 10.1084/jem.20092749

Figure 2.

Figure 2.

Influence of NK cell depletion or impaired homing of NK cells to the CNS on myelin-reactive Th1 and Th17 cell responses in the periphery and the CNS. Groups of mice (receiving mouse IgG or anti-NK1.1 mAb, and Cx3cr1+/+ or Cx3cr1−/−) were immunized with MOG/CFA/PT and sacrificed between days 12 and 20 after immunization. Lymph node (LN), spleen, and CNS cells were isolated. (A) Expansion of CD4+ T cells from lymphoid organs or CNS was assessed by CFSE dilution or BrdU incorporation assay, respectively. (B) Lymphoid or CNS cells were restimulated with MOG overnight, and IFN-γ– and IL-17–expressing CD4+ T cells were measured by intracellular staining. (C) Il-17a mRNA of sorted CD4+ T cells was measured by qRT-PCR. Data are representative of four independent experiments (n = 18–25/group). Error bars represent the means ± SEM. P = 0.007 and 0.006 for IL-17–expressing CD4+ cells (Student’s t test).