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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 1;1804(10):1988–1995. doi: 10.1016/j.bbapap.2010.06.016

Fig. 6.

Fig. 6

Time courses of nitrite-induced oxidation of Hb A0 under varied conditions. Solutions containing 30 μM Hb were rapidly mixed with 200-fold excess nitrite in 0.05 M Tris buffer at pH 7.4 in a stopped-flow instrument at room temperature. The spectral changes were monitored by a photodiode array detector over time. The absorbance changes at 577 nm plotted versus time are shown for reaction of stripped Hb A0 in air-equilibrated buffer (open circles); in O2 saturated buffer (open triangles); and in the presence of IHP in air-equilibrated buffer (closed circles).