Fig. 5.
In situ AFM images of siRNA/KL-PEI polyplexes (a-c) and siRNA/KB-PEI polyplexes (e-g) captured at different periods of time under acid-hydrolysis using pH 5.0 acetate buffer containing 150 mM NaCl. After hydrolysis, siRNA/KL-PEI polyplexes (d) and siRNA/KB-PEI polyplexes (h) were incubated in heparan sulfate (HS) solution containing 150 mM NaCl for 30 min. Cross-sectional profiles along the blue lines in the AFM images are represented. All images are 1 × 1 μm2 in scan size and 6 nm in z range.