Figure 1. Click chemistry schematic.

A. The cholesterol particle was made using a long, continuous propargyl glycolide (n=45) backbone with PEG endgroups. The azide-alkene click reaction was used to add the primary amine, secondary amine, and cholesterol ligands. B. The DMACA tagged disulfide particle is synthesized by first adding the fluorescent end group onto two shorter propargyl glycolide backbones (n=5) linked by a disulfide bond. The amine ligand was added to 80% of the available alkynes.