Paracrine FGF10 signaling promoted formation of multifocal prostate cancer resembling human PIN or prostate cancer, and exerted its effects with regional specificity.
A: Schematic representation of two adjacent grafts, one with FGF10 UGSM (1×105) and a second graft with normal UGSM (1×105) both combined with WT epithelium.
B: Histology of two adjacent grafts revealed normal prostate tubules on one side and well-differentiated prostate cancer on the other side, suggesting localized action of paracrine FGF10.
C: H&E analysis of regenerated tissue after a logarithmic reduction in the number of FGF10 UGSM revealed evidence of multifocal carcinoma. Cancer was seen in a&b, PIN adjacent to microinvasive carcinoma in c&d, multifocal PIN in e&f and predominantly normal histology was seen in g&h.
D: Expression of FGF10 indirectly detected by anti-GFP staining in areas of hyperplasia.