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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cancer Cell. 2007 Dec;12(6):572–585. doi: 10.1016/j.ccr.2007.11.002

Figure 6.

Figure 6

Serial transplantation of FGF10 induced adenocarcinoma

A: Schematic representation of transplantation experiments and histology of regenerated tubules.

B: Immunofluorescent analysis of regenerated tissue revealed formation of many WT and some red epithelial glands with a single cell layer resembling normal tubules (a&b), and multiple small red tubules adjacent to normal appearing WT glands reminiscent of well differentiated adenocarcinoma (c&d).

C: Histologic evaluation of transplanted tissue specimens revealed evidence of adenocarcinoma (a&e) and absence of contaminating FGF10 expressing stromal cells demonstrated by lack of GFP staining (b&f). The cancerous regenerated tissue revealed strong phosphotyrosine activation after withdrawal of retrovirally infected FGF10 UGSM cells (c&d, g&h).

D: Persistent strong nuclear localization of AR was observed in cancerous areas of the transplanted regenerated grafts (a&b).