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Effect of gemcitabine on the cell cycle profile of human pancreatic cancer cell lines. Cells were left untreated (CT) or incubated with increasing concentrations of gemcitabine (Gem) for 72 h. Fixed cells were analyzed by flow cytometry after propidium iodide staining. The amount of cells in the various phases of the cell cycle is indicated in percentage. Four separate experiments were performed obtaining similar results and data from one representative experiment are shown.
