FIG. 5.

Anillin is not properly localized to a contractile ring in mtsh meiotic cells. Phase-contrast (A, C, E, G) and fluorescence (B, D, F, H) micrographs of cells from wild-type (A, B, E, F) and mtsh^Z2-2620/mtsh^Z2-2620 (C, D, G, H) flies carrying a transgene encoding GFP-Anillin (Goldbach et al., 2010). GFP-anillin is nuclear in wild-type (A, B) and mtsh (C, D) primary spermatocytes. In wild-type meiotic cells (E, F), GFP-anillin localizes to the contractile ring (arrow). In mtsh meiotic cells (G, H), no GFP-anillin-containing contractile rings are seen, though GFP-anillin has redistributed from the nucleus diffusely to the cell cortex (arrow). The identical phenotype is seen in mtsh^Z2-2620/mtsh^Z2-3484 and mtsh^Z2-2620/mtsh^55207-12 transheterozygotes. Scale bars 10 μm in (A–D), (E–H).