Figure 7.

Concentration–response curves for the inhibition of the electrical field stimulation-evoked contractions of the rat (A) and guinea-pig (B) ileum myenteric plexus-longitudinal muscle (MPLM) by anandamide, arachidonylethanolamide (AEA) in the presence of ethanol or VDM-11 [(5Z,8Z,11Z,14Z)-N-(4-hydroxy-2-methylphenyl)-5,8,11,14-eicosatetraenamide]. The rat and guinea-pig MPLM were subjected to single electrical pulses of 0.5 ms duration, 110% supramaximal voltage at a frequency of 0.05 and 0.1 Hz respectively. Each curve was fitted by non-linear regression analysis. Each symbol represents the mean value of inhibition of contractions expressed as a percentage of the amplitude of the contraction measured immediately before the addition of any drug to the organ bath (n= 6). Vertical lines indicate the SEM. VDM-11 or ethanol was added 15 min before the first addition of AEA.