Figure 7.

Pharmacological inhibition or genetic deletion of cannabinoid-1 (CB₁) receptors attenuated the cisplatin-induced increased expression of reactive oxygen species-generating NADPH oxidase enzymes [NOX4(RENOX), NOX2(gp91phox)] and the inducible nitric oxide synthase (iNOS) in kidneys of mice. Cisplatin induced marked increases in expression of renal NOX4, NOX2 and iNOS mRNA 72 h following its administration to mice (A–D). These increases were attenuated by AM281 or SR141716 treatment (A and C), and also in CB₁ knockout (CB₁^−/−) mice compared with CB₁^+/+ littermates (B and D). Results are mean ± SEM of 9–10 experiments per group. *P < 0.01 versus vehicle in C57BL/6 mice (A and C) or versus CB₁^+/+ mice treated with vehicle (B and D); #P < 0.05 versus cisplatin in C57BL/6 or CB₁^+/+ mice (A/C and B/D respectively).