Figure 4.
Effects of drug treatment on contractile responses of longitudinal muscle strips from rat colon to carbachol 3 days after administration of 2,4,6-trinitrobenzene sulphonic acid enema. Effects of (A) (-)-cannabidiol (CBD) alone (5, 10, 15 and 20 mg·kg−1 i.p.), (B) Δ9-tetrahydrocannabinol (THC) alone (5, 10 and 20 mg·kg−1 i.p.) and THC in combination with 10 mg·kg−1 CBD (5 and 10 mg·kg−1 THC, T5 + C and T10 + C, respectively) and (C) sulphasalazine (300 mg·kg−1 p.o.). Contractions expressed in grams per gram of dry tissue weight. Results are expressed as mean ± SEM; n = 4–11; *P < 0.05 compared with respective vehicle group. Vehicle for CBD and THC – mixture of ethanol : cremophor : saline, 1:1:18, vehicle for sulphasalazine – 1% methylcellulose. Control group from preliminary experiments is included – phosphate-buffered saline enema, no treatment.