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(A) GFP and mycMacoco expression in lysates of nucleofected neurons. (B,C) Over-expression of mycMacoco increases surface expression of GABAARs by 71.0 ± 30 % (P < 0.05, n=5) in neurons as demonstrated by surface biotinylation and western blotting with anti-GABAAR-β3 antibodies. There is no significant difference between surface expression of GABAARs in neurons transfected with GFP compared with untransfected neurons.
