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. Author manuscript; available in PMC: 2011 Aug 18.

Published in final edited form as: Bioconjug Chem. 2010 Aug 18;21(8):1448–1454. doi: 10.1021/bc100041r

Library screening for siRNA transfection efficiency. HeLa cells stably expressing firefly and Renilla luciferase were transfected with 50 ng of firefly specific siRNA complexed at lipidoid/ siRNA ratios of 2.5:1, 5:1, 10:1 and 15:1 (wt/wt). Lipofectamine 2000 was used as instructed by the vendor. Luminescence was measured after 24 hour incubation, and the results presented relative to an untransfected control set of cells. a) Core 98 library. b) Core 100 library.

Library screening for siRNA transfection efficiency. HeLa cells stably expressing firefly and Renilla luciferase were transfected with 50 ng of firefly specific siRNA complexed at lipidoid/ siRNA ratios of 2.5:1, 5:1, 10:1 and 15:1 (wt/wt). Lipofectamine 2000 was used as instructed by the vendor. Luminescence was measured after 24 hour incubation, and the results presented relative to an untransfected control set of cells. a) Core 98 library. b) Core 100 library.