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Viability of HeLa cells post-transfection with selected lipidoids. HeLa cells were transfected with 50 ng siRNA complexed at lipidoid/ siRNA ratios of 2.5:1, 5:1, 10:1 and 15:1 (wt/wt). Lipofectamine 2000 was used as instructed by the vendor. After incubation for 24 hours, the media was replaced and CellTiter viability solution (Promega) was added. The absorbance at 490nm was read after incubating for 1 hour. Values are reported relative to an untransfected set of control cells.
