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. 2001 Feb 6;98(4):1676–1681. doi: 10.1073/pnas.041416598

Figure 2.

Figure 2

ATM-dependent activation of the IGF-IR promoter. To examine activation of the IGF-IR promoter as a function of ATM status, the indicated cells were transfected with a plasmid containing the luciferase gene driven by base pairs −2350 to +640 of the rat IGF-IR promoter region, along with a β-galactosidase expression vector as a control for transfection efficiency. The luciferase activity, as a measure of IGF-IR activation, was normalized to β-gal expression in each case. Experiments were performed in triplicate, and the results shown are the mean of three separate experiments. (A) Comparison of IGF-IR promoter activity in ATM-deficient GM5849 cells (SV40-transformed fibroblasts) and a subclone transfected with a vector expressing wild-type ATM cDNA (5849cA2), along with an unrelated wild-type SV40 transformed fibroblast cell line, GM637. (B) Comparison of IGF-IR promoter activity in primary AT fibroblasts obtained from an affected individual (GM3487) and from his heterozygous parent (GM3489).