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Slpr interacts preferentially with Rac-GTP and Cdc42-GTP. GST pull-down assay using the GTPases Rac and Cdc42, loaded with either GDP or GTP as indicated, and labeled Slpr WT or Slpr SKLC proteins from a rabbit reticulocyte lysate (35S-input). The Coomassie-Blue-stained gel (upper panels) and autoradiograph (lower panel) show expression of the fusion proteins and labeled input protein that is specifically bound, respectively.
