Fig. 5.

Substrate uptake after knockdown of genes encoding VAMP isoforms. a HL-1 cells were transfected with siRNAs 24 h after seeding. After a further 28 h the cells were serum depleted for 16 h and subsequently treated with 200 nmol/l insulin or 1 μmol/l oligomycin for 30 min (white bars, basal; grey bars, insulin; black bars, oligomycin). Afterwards, the cells were incubated with a substrate mix containing 2-deoxy-d-³H-glucose and ¹⁴C-palmitate for 10 min. Glucose uptake (b) and palmitate uptake (c) were quantified by scintillation counting of the cell lysates and corrected for the uptake component that could not be inhibited by phloretin (passive diffusion component). Data are mean values ± SEM of six independent experiments (n = 6), with triplicate measurements for each condition. Statistically different from corresponding value in control group (*p < 0.05); statistically different from corresponding basal value (^† p < 0.05). nc, non-coding