Table 1.

HPLC analysis of adenosine phosphates in HL-1 cells

Variable	Basal	Treatmenta
		Insulin	Electric-field stimulation	Oligomycin
ATP (μmol/g dry weight)	1.57 ± 0.05	1.55 ± 0.13	1.15 ± 0.22*	0.69 ± 0.03*
ADP (μmol/g dry weight)	1.77 ± 0.28	1.25 ± 0.20	1.70 ± 0.19	1.25 ± 0.05
AMP (μmol/g dry weight)	0.27 ± 0.09	0.21 ± 0.01	0.41 ± 0.10	0.31 ± 0.05
AMP/ATP	0.17 ± 0.06	0.14 ± 0.02	0.36 ± 0.18*	0.45 ± 0.09*

Lysates were freeze dried and high-energy phosphates determined by HPLC analysis

^aSerum-depleted cells were treated with 200 nmol/l insulin, electric-field stimulation (2 Hz) or 1 μmol/l oligomycin for 30 min

*p < 0.05 vs basal level